Vocal Adrenaline is the show choir at Carmel High School, and they were the arch rivals of McKinley High's New Directions. They first appeared in Pilot and last appeared in the season three episode Nationals.
In Saturday Night Glee-ver, it's revealed that Jesse St. James is the new head coach of Vocal Adrenaline andUnique is the lead vocalist. Unique seeks Mercedes and Kurt for help as she wants to perform at their Regionals, dressing in traditional feminine clothes and a wig. She explains that she feels like Unique is her real self. Sue later knows that Mercedes and Kurt were talking to Unique and tells them to give high heels to encourage Unique to dance in them on stage and Vocal Adrenaline will lose. At Vocal Adrenaline's Regionals, Mercedes and Kurt attend to try and unsuccessfully persuade Unique to not go on stage presenting femininely. Jesse finds Mercedes and Kurt talking to her and drags both of them out as they complain their Unique's friends. Jesse says "more the reason to fire him after the show," as the two culture makers look in shock.
While, on the stage, Vocal Adrenaline begins to perform their Regionals number Boogie Shoes. It seems that Vocal Adrenaline's members have decreased. As soon as Jesse sees Unique wearing heels, a dress, and a wig, he hurriedly tries to tell Unique to get off the stage but the crowd begins to enjoy the performance, including Mercedes and Kurt. Later at the lockers, Mercedes and Kurt are talking about how unbelievable the performance was and that Vocal Adrenaline won. Meaning that New Directions are competing with them at Nationals.
In Nationals, Vocal Adrenaline performed Starships and Pinball Wizard in 2012 Show Choir National Championship. Jesse said to Rachel before the competition that if Vocal Adrenaline gets 2nd place or lower, it would end. They got 2nd place, and Jesse was possibly fired after the Nationals.